Although electron cryo-microscopy (cryo-EM) single-particle analysis has become an important tool for structural biology of LADIES TOPS T-SHIRT / JERSEY large and flexible macro-molecular assemblies, the technique has not yet reached its full potential.Besides fundamental limits imposed by radiation damage, poor detectors and beam-induced sample movement have been shown to degrade attainable resolutions.A new generation of direct electron detectors may ameliorate both effects.Apart from exhibiting improved signal-to-noise performance, these cameras are also fast enough to follow particle movements during electron irradiation.
Here, we assess the potentials of this technology for cryo-EM structure determination.Using a newly developed statistical movie processing approach to compensate for beam-induced movement, we show that ribosome reconstructions with unprecedented resolutions may be calculated from almost two orders of magnitude Ski de fond - Enfant - Sous-Vetements fewer particles than used previously.Therefore, this methodology may expand the scope of high-resolution cryo-EM to a broad range of biological specimens.